To use the in operator you need to put ae in quotes:
if ("ae" in Chars){
Or you can use a variable as follows:
var valueToTest = "ae";
if (valueToTest in Chars) {
You said in a comment under another answer that you have over one hundred values to check. You don't say how you are managing those hundred, but assuming they're in an array you can use a loop:
var keyNamesToTest = ["ae", "xy", "zz", "oe"];
for (var i = 0; i < keyNamesToTest.length; i++) {
if (keyNamesToTest[i] in Chars){
document.write('yes');
// key name exists - to get the value use Chars[keyNamesToTest[i]]
}else{
document.write('no');
}
}
For the Chars object you showed with the test array I introduced you'd get a yes, two nos, and another yes.