You can simplify the regex by considering the string to be a group of two hex digits followed by an optional -, followed by 6 similar groups (i.e. if the first group had a -, the subsequent ones must too), followed by a group of 2 hex digits:
^[0-9A-Fa-f]{2}(-?)([0-9A-Fa-f]{2}\1){6}[0-9A-Fa-f]{2}$
Use of the re.I flag allows you to remove the a-f from the character classes:
^[0-9A-F]{2}(-?)([0-9A-F]{2}\1){6}[0-9A-F]{2}$
You can also simplify slightly further by replacing 0-9 by \d in the character classes (although personally I find 0-9 easier to read):
^[\dA-F]{2}(-?)([\dA-F]{2}\1){6}[\dA-F]{2}$
Demo on regex101
Sample python code:
import re
strs = ['AC-DE-48-23-45-67-AB-CD',
'ACDE48234567ABCD',
'AC-DE48-23-45-67-AB-CD',
'ACDE48234567ABC',
'ACDE48234567ABCDE']
for s in strs:
print(s + (' matched' if re.match(r'^[0-9A-F]{2}(-?)([0-9A-F]{2}\1){6}[0-9A-F]{2}$', s, re.I) else ' didn\'t match'))
Output
AC-DE-48-23-45-67-AB-CD matched
ACDE48234567ABCD matched
AC-DE48-23-45-67-AB-CD didn't match
ACDE48234567ABC didn't match
ACDE48234567ABCDE didn't match
0-9with\d.A-Fora-f.-characters, then check it matched the second case.-characters would allow something likeABCD-E-F01-23-456789to match which I don't think is OP's intent