How to use variable in one script into def of another script in python

You are trying to solve a problem that all beginner programmers run into sooner rather than later. You'll find that global variables seem to be the solution, but you can save yourself a lot of unlearning of bad habits later by looking at function parameters right away:

script1.py:
           def one(name, last, job):
               print("First Name"+name+"\n")
               print("Last Name "+last+"\n")
               print("Occupation:"+job+"\n")

script2.py:
           import script1

           name = "Rob"
           last = "Sussian"
           occupation = "Unemployee"

           print(script1.one(name, last, occupation))

Your function one() has been given three parameters, which it will expect to be assigned arguments as the function is called, which is exactly what happens in script2.py.

Note that I left the names unchanged, but the value of the variables name etc. is passed in the call, so that fact that the global variable name gets assigned to the parameter name of the function one() has nothing to do with the names. They could be named differently, and things would work as well - you can tell from job and occupation.

(Note that I changed the indentation on your function to be 4 instead of 3, which is standard for Python - a good habit as well)

(Another note, the print() around the call to the function will only print the return value of None - you don't need to print the result of a function that does the printing itself)

If you're trying to avoid passing arguments because you're operating on values that you will be reusing for other functions as well, consider using a class:

script1.py:
           class Person:
               def __init__(self, name, last, job):
                   self.name = name
                   self.last = last
                   self.job = job

               def one(self):
                   print("First Name"+self.name+"\n")
                   print("Last Name "+self.last+"\n")
                   print("Occupation:"+self.job+"\n")

script2.py:
           import script1

           rob = Person('Rob', 'Sussian', 'Unemployee')           
           rob.one()