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If I use an Arduino board to bootload a new chip, is it still possible to use the microcontroller on the board for another project, or it will only be for bootloading new chips.

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There are more ways to flash a bootloader to new AVR chip. To which you refer? If you replace the chip on Uno to burn a bootloader to it with a programmer, then it is very unclear what you are asking, because the original chip is not involved in this process.

If you ask about ArduinoISP sketch, then simply it is a sketch. You upload it as any other sketch and replace it with any other sketch.

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