Remove duplicates from csv based on conditions

Using pandas.DataFrame.groupby is pretty much the same as what you are doing using the first line in your for loop. So you can change:

for email in df['Email Address'].unique():
    subdf = df.loc[df['Email Address'] == email]
    ...

by

for email, subdf in df.groupby('Email Address'):
    ...

Now, to improve this loop:

• subdf['Registration Date Copy'].min() is not what you want since it gives you the value and you are interested by its index (as you are filtering and getting values[0] on the next line). Use argmin() instead to get the index so you can directly get the desired value with subdf['Registration Date'][<variable_name_you_stored_argmin_into>]
• You can modify a specific cell of a DataFrame using df.set_value(index, column, value), no need to use an intermediate dataframe here. Since you will drop everything but the firsts elements of each group, you can change only the ones at subdf.index[0].

This yield:

df = pd.read_csv('pra.csv')

# Sort the data by Login Date since we always need the latest
# Login date first. We're making a copy so as to keep the
# original data intact, while still being able to sort by datetime
df['Login Date Copy'] = pd.to_datetime(df['Login Date'])
df['Registration Date Copy'] = pd.to_datetime(df['Registration Date'])
df.sort_values(by='Login Date Copy', ascending=False, inplace=True)

# Change the registration date of the first duplicate to the oldest one
for email, group in df.groupby('Email Address'):
    oldest_index = group['Registration Date Copy'].argmin()
    oldest_registration = group['Registration Date'][oldest_index]
    df.set_value(group.index[0], 'Registration Date', oldest_registration)

# Drop working columns
df.drop(['Login Date Copy', 'Registration Date Copy'], axis=1, inplace=True)

# Finally, get only the first of the duplicates and output the result
df.drop_duplicates(subset='Email Address', keep='first', inplace=True)
df.to_csv('~/output.csv', index=False)

I also changed a bit the formating of the comments and used inplace=True instead of df = df.whatever().

Finally, you may consider wrapping that in a function so you can easily change the values of the input/output parameters:

import pandas as pd


def main(input_csv, output_csv):
    df = pd.read_csv(input_csv)

    # Sort the data by Login Date since we always need the latest
    # Login date first. We're making a copy so as to keep the
    # original data intact, while still being able to sort by datetime
    df['Login Date Copy'] = pd.to_datetime(df['Login Date'])
    df['Registration Date Copy'] = pd.to_datetime(df['Registration Date'])
    df.sort_values(by='Login Date Copy', ascending=False, inplace=True)

    # Change the registration date of the first duplicate to the oldest one
    for email, group in df.groupby('Email Address'):
        oldest_index = group['Registration Date Copy'].argmin()
        oldest_registration = group['Registration Date'][oldest_index]
        df.set_value(group.index[0], 'Registration Date', oldest_registration)

    # Drop working columns
    df.drop(['Login Date Copy', 'Registration Date Copy'], axis=1, inplace=True)

    # Finally, get only the first of the duplicates and output the result
    df.drop_duplicates(subset='Email Address', keep='first', inplace=True)
    df.to_csv(output_csv, index=False)


if __name__ == '__main__':
    main('pra.csv', '~/output.csv')