Usually radio buttons are used for the exclusive functionality where only one item in a group is selected and the browser will do taht for you automatically with the right HTML.
For checkboxes, you could code it with jQuery like this:
<div class="checkboxContainer">
<input id="ctl00_MainContent_mGrid_ob_mGridBodyContainer_ctl02_ctl02_ctl00_ChkID" type="checkbox"> Item 1<br>
<input id="ctl00_MainContent_mGrid_ob_mGridBodyContainer_ctl03_ctl02_ctl00_ChkID" type="checkbox"> Item 2<br>
<input id="ctl00_MainContent_mGrid_ob_mGridBodyContainer_ctl04_ctl02_ctl00_ChkID" type="checkbox"> Item 3
</div>
$("input[type='checkbox']").change(function() {
$(this).closest(".checkboxContainer").find("input[type='checkbox']").not(this).prop("checked", false);
$(this).prop("checked", true);
});
Working Demo: http://jsfiddle.net/jfriend00/hWEXx/
Here's the pure HTML way of doing exclusive radio buttons (also in that same demo):
<div class="radioGroup">
<input type="radio" name="group1">Item A<br>
<input type="radio" name="group1">Item B<br>
<input type="radio" name="group1">Item C<br>
</div>