When you put upper-left and upper-right tree view widget, pack with side=LEFT to make them packed from left to right. (You can also use side=RIGHT if you pack right one first).
Beside that, pack from top to bottom. (pack with side=TOP or omit side)
from Tkinter import *
import ttk
# In Python 3.x
#from tkinter import *
#from tkinter import ttk
root = Tk()
upper_container = Frame(root)
upper_container.pack()
left_tree = ttk.Treeview(upper_container)
left_tree.pack(side=LEFT)
right_tree = ttk.Treeview(upper_container)
right_tree.pack(side=LEFT)
lower_tree = ttk.Treeview(root)
lower_tree.pack()
root.mainloop()

UPDATE
updated version that will allow user to resize upper / lower area using PanedWindow.
from Tkinter import *
import ttk
# In Python 3.x
#from tkinter import *
#from tkinter import ttk
root = Tk()
pane = PanedWindow(orient=VERTICAL)
pane.pack()
upper_container = Frame(pane)
upper_container.pack()
left_tree = ttk.Treeview(upper_container)
left_tree.pack(side=LEFT)
right_tree = ttk.Treeview(upper_container)
right_tree.pack(side=LEFT)
lower_tree = ttk.Treeview(pane)
lower_tree.pack()
pane.add(upper_container)
pane.add(lower_tree)
root.mainloop()