If you want only the first column you could use
# Read the full file
myfile = open("myfile.txt", "r").read()
# Split file by new lines
# Will make an array looking like
# ['first_1/tsecond/tlast', 'first_2/tsecond_2/tlast_2']
lines = myfile.split('\n')
# This splits 'lines' by /t and returns only the first one
# making a new array with only the first column.
# ['first_1', 'first_2']
first_column = [line.split('/t')[0] for line in lines]
if i want to get the third column, where i have to change
# Add this below the last line
last_column = [line.split('/t')[2] for line in lines]
You can change the last_column line to something generic.
lines = [line.split('/t') for line in lines]
print(lines) : [['first_1', 'second', 'last'], ['first_2', 'second_2', 'last_2']]
print(lines[0]) : ['first_1', 'second', 'last']
print(lines[1]) : ['first_2', 'second_2', 'last_2']
print(lines[0][0]) : first
print(lines[1][1]) : second_2